Journal: Bioactive Materials

Article Title: High velocity suspension flame spraying (HVSFS) of metal doped bioceramic coatings

doi: 10.1016/j.bioactmat.2017.04.006

Figure Lengend Snippet: Hydroxyapatite coatings doped with copper, silver, bismuth and without metal dopant.

Article Snippet: Four different feedstock powders were employed in this study: hydroxyapatite (HA) , (Ca 10 (PO 4 ) 6 (OH) 2 ; Ceram GmbH, Germany), beta-tricalciumphosphate (TCP) (Ca 3 (PO 4 ) 2 ; Budenheim, Germany), a calcium potassium sodium phosphate, GB14 , with the main crystalline phase Ca 2 KNa(PO 4 ) 2 (BAM, Germany) and a bioglass (BG) with a composition of 47.3% SiO 2 , 28.6% CaO, 15.2% P 2 O 5 , 4.90% Na 2 O, 2.50% MgO and 1.5% F (Colorobbia, Italy).

Techniques:

Journal: Bioactive Materials

Article Title: High velocity suspension flame spraying (HVSFS) of metal doped bioceramic coatings

doi: 10.1016/j.bioactmat.2017.04.006

Figure Lengend Snippet: Cross sections of the non-doped implant coatings; a) hydroxyapatite; b) TCP; c) GB14; d) bioglass.

Article Snippet: Four different feedstock powders were employed in this study: hydroxyapatite (HA) , (Ca 10 (PO 4 ) 6 (OH) 2 ; Ceram GmbH, Germany), beta-tricalciumphosphate (TCP) (Ca 3 (PO 4 ) 2 ; Budenheim, Germany), a calcium potassium sodium phosphate, GB14 , with the main crystalline phase Ca 2 KNa(PO 4 ) 2 (BAM, Germany) and a bioglass (BG) with a composition of 47.3% SiO 2 , 28.6% CaO, 15.2% P 2 O 5 , 4.90% Na 2 O, 2.50% MgO and 1.5% F (Colorobbia, Italy).

Techniques:

Journal: Bioactive Materials

Article Title: High velocity suspension flame spraying (HVSFS) of metal doped bioceramic coatings

doi: 10.1016/j.bioactmat.2017.04.006

Figure Lengend Snippet: EDX mapping of a copper doped hydroxyapatite cross-section.

Article Snippet: Four different feedstock powders were employed in this study: hydroxyapatite (HA) , (Ca 10 (PO 4 ) 6 (OH) 2 ; Ceram GmbH, Germany), beta-tricalciumphosphate (TCP) (Ca 3 (PO 4 ) 2 ; Budenheim, Germany), a calcium potassium sodium phosphate, GB14 , with the main crystalline phase Ca 2 KNa(PO 4 ) 2 (BAM, Germany) and a bioglass (BG) with a composition of 47.3% SiO 2 , 28.6% CaO, 15.2% P 2 O 5 , 4.90% Na 2 O, 2.50% MgO and 1.5% F (Colorobbia, Italy).

Techniques:

Journal: Scientific Reports

Article Title: The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

doi: 10.1038/s41598-017-03086-0

Figure Lengend Snippet: Hydroxyapatite particle characterization.

Article Snippet: Hydroxyapatite particles of three different sizes and two different morphologies were purchased from Plasma Biotal Limited (Table ).

Techniques:

Journal: Scientific Reports

Article Title: The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

doi: 10.1038/s41598-017-03086-0

Figure Lengend Snippet: Hydroxyapatite particle size and shape dictates cytokine production by murine BMDCs. BMDCs (0.625 × 10 6 cells · ml −1 ) from C57BL/6 mice were stimulated with concentrations ranging from 0.1 mg · ml −1 to 1 mg · ml −1 alone or after priming with LPS (1 ng · ml −1 ) for 3 h. Supernatants were collected 24 hours later and tested for IL-1β ( A , C , D ) by ELISA. Unprimed cells were collected and stained with LIVE/DEAD ® fixable Aqua dead cell stain and CD11c + and cell death was assessed by flow cytometry ( B ). Representative SEM pictures of HA particles ( A ). Results are mean cytokine concentrations (±SD) for triplicate samples (( A ) vs LPS alone; ( D ) vs Total, NS p > 0.05, *p < 0.05 and ***p < 0.001). Data are representative of three independent experiments. ( B ) Results are pooled from three independent experiments. Error bars show means ± SEM for triplicate samples (vs media *p < 0.05); NS: non-significant.

Article Snippet: Hydroxyapatite particles of three different sizes and two different morphologies were purchased from Plasma Biotal Limited (Table ).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Flow Cytometry

Journal: Scientific Reports

Article Title: The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

doi: 10.1038/s41598-017-03086-0

Figure Lengend Snippet: ( A ) Schematic of hydroxyapatite particles. ( B ) HA internalization by BMDCs. SEM images of BMDCs incubated with N 5 , R 20 and S 20 particles for 18 h. ( C ) Confocal fluorescence microscopy images of BMDCs incubated with FITC-labelled (green) S 0.1 , N 5 , R 20 and S 20 for 24 h to visualize the individual internalized particles. Membrane staining with phalloidin–TRITC (red) and nuclear staining with DAPI (blue).

Article Snippet: Hydroxyapatite particles of three different sizes and two different morphologies were purchased from Plasma Biotal Limited (Table ).

Techniques: Incubation, Fluorescence, Microscopy, Membrane, Staining

Journal: Scientific Reports

Article Title: The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

doi: 10.1038/s41598-017-03086-0

Figure Lengend Snippet: Hydroxyapatite particle shape dictates cytokine production by murine BMDMs. BMDMs (1.0 × 10 6 cells · ml −1 ) from C57BL/6 mice were stimulated with particles at concentrations ranging from 0.4 mg · ml −1 to 1 mg · ml −1 alone or after priming with LPS (10 ng · ml −1 ) for 3 h. Supernatants were collected 24 hours later and tested for ( A ) IL-1β secretion by ELISA or ( B ) LDH release to assess cell viability. ( A ) Results are mean cytokine concentrations (±SD) for triplicate samples (vs LPS alone NS p > 0.05, *p < 0.05 and ***p < 0.001). Data are representative of three independent experiments. ( B ) Error bars show means ± SD for triplicate samples (vs media *p < 0.05 and ***p < 0.001). Results are representative of two independent experiments.

Article Snippet: Hydroxyapatite particles of three different sizes and two different morphologies were purchased from Plasma Biotal Limited (Table ).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

doi: 10.1038/s41598-017-03086-0

Figure Lengend Snippet: Hydroxyapatite particle-induced IL-1β production is NLRP3 dependent. ( A ) BMDCs (0.625 × 10 6 cells · ml −1 ) from C57BL/6 or NLRP3 −/− mice were stimulated with HA particles at concentrations ranging from 0.2 mg · ml −1 to 1 mg · ml −1 after priming with LPS (1 ng · ml −1 ) for 3 h. Supernatants were collected after 24 hours and IL-1β levels were measured by ELISA. Data are shown as mean ± SD for triplicate samples (vs WT ***p < 0.001; NS: non-significant). Results are representative of three independent experiments. ( B ) BMDCs (0.625 × 10 6 cells · ml −1 ) from female C57BL/6 mice were primed with LPS (1 ng · ml −1 ) for 3 h before stimulating with 0.8 mg · ml −1 of HA particles; cells were incubated with the lysosome acidification inhibitor bafilomycin A (62.5–250 nM), the cathepsin B inhibitor CA-074-Me (2.5–10 µM) and with the ROS inhibitor DPI (5–20 µM) 1 h before particulate addition. Supernatants were collected after 24 hours and IL-1β levels were measured by ELISA. Data are shown as mean ± SD for triplicate samples. Results are representative of three independent experiments (vs no inhibitor ***p < 0.001).

Article Snippet: Hydroxyapatite particles of three different sizes and two different morphologies were purchased from Plasma Biotal Limited (Table ).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation